Analytical Methods
Analytical method portfolio
GlycoThera develops, validates and applies state-of-the-art techniques and analytical methods for its client.The following list provides with an overview about the analytical methods routinely performed at GlycoThera:
Capillary electrophoresis
- Capillary zone electrophoresis (CZE)
- Capillary isoelectric focusing (cIEF)
- Capillary electrophoresis sodium dodecyl sulfate (CE-SDS; reduced and non-reduced)
Circular dichroism
Isoelectric focusing (IEF)
Immuno affinity chromatography techniques
High performance liquid chromatography (HPLC)
- Reverse Phase (RP)
- Ion Exchange (IEX)
- Size Exclusion (SEC)
Mass spectrometry
- Electrospray Ionisation (ESI)
- Matrix-Assisted Laser-Desorption/Ionisation (MALDI)
- MS-MS sequencing of peptides
- MS-MS sequencing of oligosaccharides
Monoclonal antibody (mAb) purification (and quantification)
- Protein A
- Protein G
Peptide mapping (RP-HPLC; optionally with fraction collection)
- with UV detection
- and/or MS / MS-MS detection
Quantitative oligosaccharide mapping
- High-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD)
- 2-aminobenzamide (2-AB)-labelled glycans + fluorescence detection
Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)
- one-dimensional (1D)
- two-dimensional (2D)